Amphibians

Includes frogs, salamanders, mudpuppies

1. Acceptable
• Sodium pentobarbital
• 120 mg/kg Intracoelomic, IV, Lymph sacs
• Controlled substance
• Inhalants
• In appropriate species
• Requires a hood/ventilation
• Toxic to personnel
• Pregnant persons should avoid
• Should be less than 2 ppm in room
• Requires some other method of euthanasia to ensure death such as removal of vital organ, exsanguination, or open chest
• CO2
• CO
• MS 222
• 1-2 grams/liter bath (Frogs, Salamanders)
• Can take 10-20 minutes for effect
• 200 mg/kg SC/IP
• Benzocaine hydrochloride
• 500 mg/L bath (Frogs, Salamanders)
• Double pithing
2. Conditionally Acceptable
• Penetrating captive bolt
• Gunshot
• Stunning and decapitation
• Decapitation and pithing
3. Unacceptable
• Freezing without general anesthesia

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